Comparison of fecal culture and F57 real-time polymerase chain reaction for the detection of Mycobacterium avium subspecies paratuberculosis in Swiss cattle herds with a history of paratuberculosis
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منابع مشابه
Comparison of fecal culture and F57 real-time polymerase chain reaction for the detection of Mycobacterium avium subspecies paratuberculosis in Swiss cattle herds with a history of paratuberculosis
BACKGROUND Bovine paratuberculosis is an incurable chronic granulomatous enteritis caused by Mycobacterium avium subspecies paratuberculosis (MAP). The prevalence of MAP in the Swiss cattle population is hard to estimate, since only a few cases of clinical paratuberculosis are reported to the Swiss Federal Food Safety and Veterinary Office each year.Fecal samples from 1,339 cattle (855 animals ...
متن کاملIdentification of Mycobacterium avium subspecies paratuberculosis by Polymerase Chain Reaction in Blood and Semen of a Bull with Clinical Paratuberculosis
Mycobacterium avium subspecies paratuberculosis was identified by nested polymerase chain reaction in the blood and semen of a 4year-old Holstein breeding bull that exhibited clinical signs of paratuberculosis and was serologically positive for the organism by agar gel immunodiffusion and ELISA, although three fecal cultures were negative for the organism. The animal subsequently died, but a ne...
متن کاملDetection of Mycobacterium avium subspecies paratuberculosis using nested polymerase chain reaction (nPCR)
Mycobacterium avium subspecies paratuberculosis, the cause of paratuberculosis (Johne’s disease) is a chronic debilitating infection in ruminants. The disease is one of the most widespread bacterial infections of ruminants throughout the world. Recently, the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the prese...
متن کاملCorrelation between Herrold egg yolk medium culture and real-time quantitative polymerase chain reaction results for Mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental samples.
Real-time quantitative polymerase chain reaction (qPCR) testing for Mycobacterium avium subspecies paratuberculosis (MAP) in fecal samples is a rapid alternative to culture on Herrold egg yolk medium (HEYM), the traditional antemortem reference test for MAP. Although the sensitivity and specificity of these 2 tests have been estimated based on dichotomized test results, the correlation between ...
متن کاملGenomic Detection of Mycobacterium Avium Subspecies Paratuberculosis in Blood Samples of Patients with Inflammatory Bowel Disease in Southern Iran
Background: Inflammatory bowel disease (IBD), of which Crohn’s disease (CD) and ulcerative colitis (UC) are the two main clinicopathological subtypes, is a group of digestive system diseases of unknown etiology. Risk factors for IBD are environmental factors, genetics, and immune system agents. Mycobacterium avium subspecies paratuberculosis (MAP) is one of the most important infectious factors...
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ژورنال
عنوان ژورنال: Acta Veterinaria Scandinavica
سال: 2014
ISSN: 1751-0147
DOI: 10.1186/s13028-014-0068-9